Biphasic Effects of FGF2 on Odontoblast Differentiation Involve Changes in the BMP and Wnt Signaling Pathways
Life Sciences | Medicine and Health Sciences
Odontoblast differentiation during physiological and reparative dentinogenesis is dependent upon multiple signaling molecules, including Fibroblast Growth Factors (FGFs), Bone Morphogenetic Proteins (BMPs) and Wingless/Integrated (Wnt) ligands. Recent studies in our laboratory showed that continuous exposure of primary dental pulp cultures to FGF2 exerted biphasic effects on the expression of markers of dentinogenesis. In the present study we examined the possible involvement of the BMP and Wnt signaling pathways in mediating the effects of FGF2 on dental pulp cells. Our results showed that stimulatory effects of FGF2 on dentinogenesis during the proliferation phase of growth were associated with increased expression of the components of the BMP (Bmp2, Dlx5, Msx2, Osx) and Wnt (Wnt10a, Wisp2) pathways, and decreased expression of an inhibitor of the Wnt signaling, Nkd2. Further addition of FGF2 during the differentiation/mineralization phase of growth resulted in decreased expression of components of the BMP signaling (Bmp2, Runx2, Osx) and increased expression of inhibitors of the Wnt signaling (Nkd2, Dkk3). This suggests that both BMP and Wnt pathways may be involved in mediating the effects of FGF2 on dental pulp cells.
Sagomonyants, Karen and Mina, Mina, "Biphasic Effects of FGF2 on Odontoblast Differentiation Involve Changes in the BMP and Wnt Signaling Pathways" (2014). UCHC Articles - Research. 264.
Connect Tissue Res. Author manuscript; available in PMC 2015 Apr 21. Published in final edited form as: Connect Tissue Res. 2014 Aug; 55(0 1): 53–56. doi: 10.3109/03008207.2014.923867 PMCID: PMC4404504 NIHMSID: NIHMS679317