Chemical and Biological Triggers for the Improved Intercalation and Release of Glucose Oxidase (GO) in the Galleries of α-Zirconium Phosphate Nanolayers for a Noninvasive Oral Alternative to Insulin Drug Therapies
Date of Completion
Challa V. Kumar
Noninvasive treatment alternatives for diseases from diabetes to hypercholerestemia are at the cutting edge of research. Although many noninvasive drug delivery routes, including oral nasal, buccal, dermal, and pulmonary have been investigated no one route has been fully approved for patient use by the Food and Drug Administration (FDA) standards as an alternative to current needle dependent subcutaneous insulin delivery. 10 12 This thesis focuses on two novel oral protein drug delivery systems α-Zr(IV) phosphate (α-ZrP)-protein-Divalent Metal Ion and α-Zr(IV) phosphate (α-ZrP)-protein- cationized Bovine Serum Albumin BSA. Glucose oxidase (GO) serves as a model protein in this thesis for noninvasive alternatives to lowering blood sugar in type one and two diabetics. However, instead of encapsulating GO, an oral drug treatment for diabetes, alternative proteins can also be encapsulated as oral drug treatments for ailments such as hypercholesterolemia This experiment systematically tests what ionized version of BSA (native BSA-TEPA (-25), BSA-TEPA (0), BSA-TEPA (-13), BSA-TEPA (+5), or divalent metal ion (Mg (II), Ca (II), or Ba (II)) intercalated into ZrP results in maximal GO binding and release in the intestinal region of the GI tract.
Afrede, Momina Kamal, "Chemical and Biological Triggers for the Improved Intercalation and Release of Glucose Oxidase (GO) in the Galleries of α-Zirconium Phosphate Nanolayers for a Noninvasive Oral Alternative to Insulin Drug Therapies" (2013). Honors Scholar Theses. 328.