Rhodanese activity from crude extracts of Thiobacillus sp. strain IV-85 was demonstrated in polyacrylamide gels after incubation in the reaction mixture by staining with dichloroindophenol in the presence of methylphenazonium methosulfate. The sensitivity of the staining system was found to be 8 x 10 moles of sulfite.
Murphy, J R; Frankenfeld, J; Calvert, J; and Tilton, R C, "Demonstration of Rhodanese Activity in Polyacrylamide Gels." (1972). SoM Articles. 17.
Originally published in : Applied Microbiology. 1972 Aug;24(2):283-285.