Date of Completion
Dr. Challa V. Kumar; Dr. Xudong Yao
Field of Study
Master of Science
A simple method is reported to fabricate gold arrays featuring microwells surrounding 8-electrodes from gold compact discs (CDs) for less than $0.2/chip. Integration of these disposable gold CD array chips with microfluidics provided inexpensive immunoarrays that were used to measure a cancer biomarker protein quickly at high sensitivity. The gold CD sensor arrays were fabricated using thermal transfer of laserjet toner from a computer-printed pattern followed by selective chemical etching. Sensor elements had electrochemically addressable surface area of 0.42 mm2 with RSD -1 (385 aM) with a linear response with log of IL-6 concentration from 10 to 1300 fg mL-1. These easily fabricated, ultrasensitive, microfluidic immunosensors should be readily adapted for sensitive detection of multiple biomarkers for cancer diagnostics.
Tang, Chi, "Fabrication of Immunosensor Microwell Arrays from Gold Compact Discs for Detection of Cancer Biomarker Proteins" (2013). Master's Theses. 433.
Dr. James F. Rusling