Date of Completion
Spring 5-1-2014
Thesis Advisor(s)
Michael A. Lynes
Honors Major
Biological Sciences
Disciplines
Biology | Literature in English, North America, Ethnic and Cultural Minority
Abstract
Humans are exposed to a variety of toxic substances in their day-to-day activities that may have adverse health effects if not properly identified. An integrated dual-mode instrument that combines grating-coupled surface plasmon resonance (GCSPR) and grating-coupled surface plasmon coupled emissions (GCSPCE) should allow for the efficient detection of toxic analytes in a high-content system using gold-coated superparamagnetic nanobeads. The goal of this study is to validate the binding characteristics of the reagents intrinsic to this innovative assay methodology. Competition enzyme-linked immunosorbent assays (ELISA) and surface plasmon resonance (SPR) assays were conducted on NNK toxicants and SEB toxins, which were the prototype toxicant and toxin being analyzed.
Recommended Citation
Ollayos, William B., "Detection Methods for Small Analytes: Improvement of Novel SPR Technology Detection of Toxins and Toxicants" (2014). Honors Scholar Theses. 356.
https://digitalcommons.lib.uconn.edu/srhonors_theses/356