Date of Completion
5-6-2016
Embargo Period
8-30-2021
Advisors
Leighton Core, Judy Brown
Field of Study
Molecular and Cell Biology
Degree
Master of Science
Open Access
Campus Access
Abstract
While the loss of genomic integrity is a widespread hallmark of cancer cells, little is known about the mechanisms that drive chromosome rearrangements and the sequence of events by which the genome increases in oncogenic potential. Here, we integrate data gathered at different points throughout the process of tumorigenesis, ranging from healthy/susceptible tissue to metastatic lesion with precancerous and neoplastic intermediates. We show through RNA sequencing, bisulfite sequencing, and targeted genomic sequencing that early changes in transcription and DNA methylation at the precancerous stage precede widespread genomic volatility and may predispose certain genomic regions to malignant chromosomal instability.
Recommended Citation
Smalec, Brendan M., "Defining Early Epigenetic Drivers of Malignancy: Hypomethylation of Repetitive Elements in Precancerous Tissues Precedes Targeted Chromosome Instability in Primary Tumors and Widespread Genomic Instability in Metastases" (2016). Master's Theses. 970.
https://digitalcommons.lib.uconn.edu/gs_theses/970
Major Advisor
Rachel O'Neill